Scientific Publications
Comparison of Humoral Immune Response to SARS-CoV-2 Infection and/or Immunization in Primary Immunodeficient, Rheumatic and Healthy Individuals
Cell Reports Methods, Rapid detection of an Ebola biomarker with optical microring resonators
Qavi et al. describe a method using microring resonator sensors to detect Ebola virus (EBOV) soluble glycoprotein (sGP) in serum. sGP is a promising biomarker for the prognosis of EBOV infection and, coupled with this rapid detection method, could lead to a faster...
6 month serologic response to the Pfizer-BioNTech COVID-19 vaccine among healthcare workers
Jason Cham, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing,# 1 , 2 Amitabh C. Pandey, Writing – review &...
Pre-COVID-19 Immunity to Common Cold Human Coronaviruses Induces a Recall-Type IgG Response to SARS-CoV-2 Antigens Without Cross-Neutralisation
The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against de novo COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG)...
BNT162b2 vaccine-induced humoral and cellular responses against SARS-CoV-2 variants in systemic lupus erythematosus
Abstract Objectives Our aim was to evaluate systemic lupus erythematosus (SLE) disease activity and SARS-CoV-2-specific immune responses after BNT162b2 vaccination. Methods In this prospective study, disease activity and clinical assessments were recorded...
Target specific serologic analysis of COVID-19 convalescent plasma
Abstract This study compared the performance of four serology assays for Coronavirus Disease 2019 (COVID-19) and investigated whether COVID-19 disease history correlates with assay performance. Samples were tested at Northshore using the Elecsys Anti-SARS-CoV-2 (Roche...
IgA dominates the early neutralizing antibody response to SARS-CoV-2
Abstract Humoral immune responses are typically characterized by primary IgM antibody responses followed by secondary antibody responses associated with immune memory and composed of IgG, IgA, and IgE. Here, we measured acute humoral responses to SARS-CoV-2, including...
Anti-MDA5 juvenile idiopathic inflammatory myopathy: a specific subgroup defined by differentially enhanced interferon-α signalling
Abstract Objectives JDM and juvenile overlap myositis represent heterogeneous subtypes of juvenile idiopathic inflammatory myopathy (JIIM). Chronic evolution can occur in up to 60% of cases, and morbidity/mortality is substantial. We aimed to describe the clinical,...
Détection des anticorps anti-MDA5 par une nouvelle technique simple et rapide
Introduction Une pneumopathie interstitielle diffuse (PID) rapidement évolutive de pronostic sombre peut révéler une dermatomyosite amyopathique avec anticorps anti-MDA5. Les signes extra-thoraciques peuvent être très discrets et les tests immunologiques actuels sont...
Detection in whole blood of autoantibodies for the diagnosis of connective tissue diseases in near patient testing condition
Read details here: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202736 Makoto Miyara , Jean-Luc Charuel, Sasi Mudumba, Alice Wu, Pascale Ghillani-Dalbin, Zahir Amoura , Rufus W. Burlingame , Lucile Musset. Aug 2018
Systemic Lupus Erythematosus with Anti-DSF70 Antibodies
Presented at the 11th International Congress of Autoimmunity, May 16th-20th, 2018, Lisbon, Portugal.
Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time
Abstract Photonic ring resonance is a property of light where in certain circumstances specific wavelengths are trapped in a ring resonator. Sensors based on silicon photonic ring resonators function by detecting the interaction between light circulating inside the...
Near Patient Detection of anti MDA5 Antibodies Using Photonic Ring Immunoassays 2017
Presented at the 2017 ATS Conference, May 24, 2017, Washington D.C.
Near Patient Anti-Nuclear Antibody Multiplex Testing Using Whole Blood for the Diagnosis of Connective Tissue Diseases in a Tertiary Care Center
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Application of a Novel Anti-Nuclear Antibody Multiplex Test Using Fingerstick and Venous Whole Blood in a Rheumatology Clinic
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Multiplexed cancer biomarker detection using chip-integrated silicon photonic sensor arrays
Abstract The analysis of disease-specific biomarker panels holds promise for the early detection of a range of diseases, including cancer. Blood-based biomarkers, in particular, are attractive targets for minimally-invasive disease diagnosis. Specifically, a panel of...
Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform
Abstract Objectives We report the development of an optical immunosensor for the detection of monocyte chemotactic protein 1 (MCP-1) in serum samples. MCP-1 is a cytokine that is an emerging biomarker for several diseases/disorders, including ischemic...
Emerging Biosensing Approaches for microRNA Analysis
Biosensor Methods miRNAs have been a focus of biosensor development over the past decade, with many micro- and nanoscale sensing technologies being applied to this class of analytes. Benefits of many of these schemes lie in their potential for diagnostics at the point...
Characterization of Anti-Nuclear Antibody (ANA) Signatures in Murine Models of Lupus Using Genalyte Maverick Technology
Presented at the 2015 ACR/ARHP Annual Meeting, November 6-11, 2015, San Francisco CA
Detection of all ADA subclass and isotypes, including monovalent IgG4, on a Multiplex Photonic Ring Immunoassay
Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays
Abstract Extracellular signaling is commonly mediated through post-translational protein modifications that propagate messages from membrane-bound receptors to ultimately regulate gene expression. Signaling cascades are ubiquitously intertwined, and a full...
Magnetically-actuated, bead-enhanced silicon photonic immunosensor
Abstract Magnetic actuation has been introduced to an optical immunosensor technology resulting in improvements in both rapidity and limit of detection for an assay quantitating low concentrations of a representative protein biomarker. For purposes of demonstration,...
An organophosphonate strategy for functionalizing silicon photonic biosensors
Abstract Silicon photonic microring resonators have established their potential for label-free and low-cost biosensing applications. However, the long-term performance of this optical sensing platform requires robust surface modification and biofunctionalization....
Label-free, multiplexed biomolecular analysis using arrays of silicon photonic microring resonators
Abstract We have developed a broadly applicable biosensing platform based upon chip-integrated silicon photonic microcavities. Large arrays of microring resonators are robustly fabricated using standard semiconductor processing methods on silicon-on-insulator wafers....
High-Q optical sensors for chemical and biological analysis
Introduction Optical sensors represent a vitally important class of analytical tools that have been used to provide chemical information ranging from analyte concentration and binding kinetics to microscopic imaging and molecular structure. Optical sensors utilize a...
Label-free virus detection using silicon photonic microring resonators
Abstract Viruses represent a continual threat to humans through a number of mechanisms, which include disease, bioterrorism, and destruction of both plant and animal food resources. Many contemporary techniques used for the detection of viruses and viral infections...
Isothermal Discrimination of Single-Nucleotide Polymorphisms via Real-Time Kinetic Desorption and Label-Free Detection of DNA Using Silicon Photonic Microring Resonator Arrays
Abstract We report a sensitive, label-free method for detecting single-stranded DNA and discriminating between single nucleotide polymorphisms (SNPs) using arrays of silicon photonic microring resonators. In only a 10 min assay, DNA is detected at subpicomole levels...
Anti-DNA:RNA Antibodies and Silicon Photonic Microring Resonator Arrays Enable the Ultrasensitive, Multiplexed Detection of microRNAs
Abstract In this paper, we present a method for the ultrasensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes, using a silicon photonic microring resonator array transduction platform. Microring resonator...
Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads
Abstract We demonstrate a three-step assay on a silicon photonic microring resonator-based detection platform that enables the quantitation of the cardiac biomarker C-reactive protein (CRP) over a dynamic range spanning six orders of magnitude. Using antibody-modified...
New Multiparameter Bioanalytical Technologies for Applications in Personalized Medicine, Drug Discovery, and Fundamental Biology
Abstract The ability to determine expression levels of multiple genetic and/or proteomic signatures simultaneously from the same sample volume is of tremendous importance in a wide range of biomedical applications including disease diagnostics, drug discovery, and...
DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format
Abstract High affinity capture agents against protein targets are essential components for immunoassays, regardless of specific analysis format. Here, we describe the use of DNA-encoded antibodies for rapidly screening the kinetic and equilibrium binding properties of...
Multiplexed inkjet functionalization of silicon photonic biosensors
Abstract The transformative potential of silicon photonics for chip-scale biosensing is limited primarily by the inability to selectively functionalize and exploit the extraordinary density of integrated optical devices on this platform. Silicon biosensors, such as...
Silicon photonic microring resonator arrays for scalable and multiplexable bioanalysis
Read more: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/7888/1/Silicon-photonic-microring-resonator-arrays-for-scalable-and-multiplexable-bioanalysis/10.1117/12.880492.full?SSO=1
Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform
Abstract Silicon photonic microring resonators have emerged as a sensitive and highly multiplexed platform for real-time biomolecule detection. Herein, we profile the evanescent decay of device sensitivity towards molecular binding as a function of distance from the...
Multiplexed Evaluation of Capture Agent Binding Kinetics Using Arrays of Silicon Photonic Microring Resonators
Abstract High affinity capture agents recognizing biomolecular targets are essential to the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously...
Photonics-on-a-chip: recent advances in integrated waveguides as enabling detection elements for real-world, lab-on-a-chip biosensing applications
Abstract By leveraging advances in semiconductor microfabrication technologies, chip-integrated optical biosensors are poised to make an impact as scalable and multiplexable bioanalytical measurement tools for lab-on-a-chip applications. In particular, waveguide-based...
Efficient Bioconjugation of Protein Capture Agents to Biosensor Surfaces Using Aniline-Catalyzed Hydrazone Ligation
Abstract Aniline-catalyzed hydrazone ligation between surface-immobilized hydrazines and aldehyde-modified antibodies is shown to be an efficient method for attaching protein capture agents to model oxide-coated biosensor substrates. Silicon photonic microring...
Sizing up the future of microRNA analysis
Abstract In less than 20 years, our appreciation for micro-RNA molecules (miRNAs) has grown from an original, curious observation in worms to their current status as incredibly important global regulators of gene expression that play key roles in many transformative...
Multiplexed detection and label-free quantitation of microRNAs using arrays of silicon photonic microring resonators
Read the details here: https://pubmed.ncbi.nlm.nih.gov/20491114/
Silicon Photonic Microring Resonators for Quantitative Cytokine Detection and T-Cell Secretion Analysis
Abstract The ability to perform multiple simultaneous protein biomarker measurements in complex media with picomolar sensitivity presents a large challenge to disease diagnostics and fundamental biological studies. Silicon photonic microring resonators represent a...
Quantitative, Label-Free Detection of Five Protein Biomarkers Using Multiplexed Arrays of Silicon Photonic Microring Resonators
Abstract Because of the inherent complexity of biochemical pathways commonly altered in disease states, it has become accepted that multiplexed analyses can provide a more informative biomolecular understanding of disease onset and progression. Importantly, compared...
Label-Free Quantitation of a Cancer Biomarker in Complex Media Using Silicon Photonic Microring Resonators
Abstract Recent advances in label-free biosensing techniques have shown the potential to simplify clinical analyses. With this motivation in mind, this paper demonstrates for the first time the use of silicon-on-insulator microring optical resonator arrays for the...
Label-Free Technologies for Quantitative Multiparameter Biological Analysis
Abstract In the post-genomic era, information is king and information-rich technologies are critically important drivers in both fundamental biology and medicine. It is now known that single-parameter measurements provide only limited detail and that quantitation of...
